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藥靶細胞株 > GPCR細胞株 > CBP71404CXCR2/β-Arrestin/CHO GPCR細胞

CXCR2/β-Arrestin/CHO GPCR細胞
名稱 CXCR2/β-Arrestin/CHO GPCR細胞
型號 CBP71404
報價
特點 CXCR2/β-Arrestin/CHO
  • 詳細內容
CBP71404
I. Background
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein-activated second messenger system (Gi/o-coupled[6]). This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. In addition, it binds ligands CXCL2, CXCL3, and CXCL5.

II. Introduction
Host Cell:

CHO

Stability:20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:F12k+10%FBS+5ug/ml puromycin+5ug/ml blasticidin
Mycoplasma Status:Negative
Storage:Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for CXCR2 receptor

Transducer:β-arrestin

Ⅲ. Description of Host Cell Line
Organism:Hamster
Tissue:Ovary
Morphology: Epithelial
Growth Properties: Adherent

Ⅳ. Representative Data

CBP71404 流式.png

Figure 1. Recombinant CXCR2/β-Arrestin/CHO constitutively expressing CXCR2.


CBP71404 數據圖1.jpg

Figure 2.Dose response of Human IL-8(CXCL8) in CXCR2 β-Arrestin CHO-K1 Cell Line (C26).

CBP71404 數據圖2.jpg

Figure 3.Inhibition of hlL-8(CXCL8)-induced Beta-Arrestin Recruitment By IL-8 Neutralization Ab in CXCR2 Beta-Arrestin CHO-K1 Cells (C26).


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